Composite
Part:BBa_M36828:Design
Designed by: Leah Chase Group: Stanford BIOE44 - S11 (2014-10-22)
Zinc Sensor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 180
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 180 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 176
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 180
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 180
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to enter silent mutations to change the DNA sequence without affecting the amino acid sequence because there were palindromes greater than 10 bp in every basic part.
Source
The genomic sequence comes from an E. Coli protein (ZntR) found in basic parts within the registry.