Composite

Part:BBa_M36828:Design

Designed by: Leah Chase   Group: Stanford BIOE44 - S11   (2014-10-22)


Zinc Sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to enter silent mutations to change the DNA sequence without affecting the amino acid sequence because there were palindromes greater than 10 bp in every basic part.


Source

The genomic sequence comes from an E. Coli protein (ZntR) found in basic parts within the registry.

References